Real-Time TaqMan PCR for Yersinia enterocolitica Detection Based on the ail and foxA Genes

Real - time TaqMan PCR for Yersinia enterocolitica detection based on 1 the ail and foxA genes

1 Corresponding author. Mailing address: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 155 Changbai Road Changping District 102206, Beiing, People’s Republic of China. Phone: 8610 58900758. Fax: 8610-58900070. E-mail: [email protected], [email protected] a ...

Real-time TaqMan PCR for Yersinia enterocolitica detection based on the ail and foxA genes.

Yersinia enterocolitica, a cause of emerging enteric infections, is a foodborne pathogen associated with various enteric and systemic syndromes, e.g., diarrhea, enteritis, and enterocolitis. Therefore, the detection of this pathogen has important significance. Previous real-time PCR for detection of Y. enterocolitica was primarily based on the ail gene; biotype 1A nonpathogenic strains were not...

Yersinia Enterocolitica Real Time PCR Kit

The detection of Yersinia enterocolitica in surface water by quantitative PCR amplification of the ail and yadA genes.

Yersinia enterocolitica has been detected in surface water, and drinking untreated water is a risk factor for infection. PCR-based methods have been used to detect Y. enterocolitica in various sample types, but quantitative studies have not been conducted in water. In this study, quantitative PCR (qPCR)-based methods targeting the Yersinia virulence genes ail and yadA were used to survey the Gr...

Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium

Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...